Near Infrared Microspectroscopy, Fluorescence Microspectroscopy, Infrared Chemical Imaging and High-Resolution Nuclear Magnetic Resonance Analysis of Soybean Seeds, Embryos and Single Cells

Chemical analysis of soybean seeds, somatic embryos and single cells were carried out by Fourier Transform Infrared (FT-IR), Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fluorescence and High-Resolution NMR (HR-NMR). The first FT-NIR chemical images of biological systems approaching 1 micron (1μ) resolution are presented here. Chemical images obtained by FT-NIR and FT-IR Microspectroscopy are presented for oil in soybean seeds and somatic embryos under physiological conditions. FT-NIR spectra of oil and proteins were obtained for volumes as small as 2μ3. Related, HR-NMR analyses of oil contents in somatic embryos are also presented here with nanoliter precision. Such 400 MHz 1H NMR analyses allowed the selection of mutagenized embryos with higher oil content (e.g. ~20%) compared to non-mutagenized control embryos. Moreover, developmental changes in single soybean seeds and/or somatic embryos may be monitored by FT-NIR with a precision approaching the picogram level. Indeed, detailed chemical analyses of oils and phytochemicals are now becoming possible by FT-NIR Chemical Imaging/ Microspectroscopy of single cells. The cost, speed and analytical requirements of plant breeding and genetic selection programs are fully satisfied by FT-NIR spectroscopy and Microspectroscopy for soybeans and soybean embryos. FT-NIR Microspectroscopy and Chemical Imaging are also shown to be potentially important in functional Genomics and Proteomics research through the rapid and accurate detection of high-content microarrays (HCMA). Multi-photon (MP), pulsed femtosecond laser NIR Fluorescence Excitation techniques were shown to be capable of Single Molecule Detection (SMD). Therefore, such powerful techniques allow for the most sensitive and reliable quantitative analyses to be carried out both in vitro and in vivo. Thus, MP NIR excitation for Fluorescence Correlation Spectroscopy (FCS) allows not only single molecule detection, but also molecular dynamics and high resolution, submicron imaging of femtoliter volumes inside living cells and tissues. These novel, ultra-sensitive and rapid NIR/FCS analyses have numerous applications in important research areas, such as: agricultural biotechnology, food safety, pharmacology, medical research and clinical diagnosis of viral diseases and cancers

Near Infrared Reflectance Spectroscopy (NIRS) Determination of Isoflavone Contents for Selected Soybean Accessions

Soybean isoflavones are of considerable interest in relation to their possible health effects in human diets. The rapid and economical determination of soybean isoflavone concentrations is essential for the investigation and development of soybean health foods as well as the selection of soybean seeds with optimal isoflavone levels for such foods. Fourier transforms near infrared reflectance spectroscopy (FT-NIRS) calibrations were developed for the rapid and cost-effective analysis of isoflavones in soybean seeds. FT-NIRS measurements were carried out in quadruplicate for 50 soybean lines selected from the USDA Soybean Germplasm Collection. The selected soybean seeds provided a wide range of isoflavone concentrations (from 0.3 to 6.0 mg/g) that is necessary for development of high-quality calibrations. Laboratory reference values of isoflavone composition were obtained by HPLC analysis of extracted soybean powders. Single soybean seeds were selected for each standard sample and were cut in half in order to avoid screening of the isoflavones NIR absorption bands by the seed coat. For comparison purposes, measurements were also made on soybean powders of the same samples. FT -NIR spectra were collected with a spectral range from 4000 to 12000 cm-1 at a resolution of 8 cm-1 on a Perkin-Elmer Spectrum one NTS spectrometer model. This spectrometer is optimized for high sensitivity analysis of single seed composition, being equipped with an NIRA, integrating sphere accessory and an extended range InGaAs detector

Magnification of spin Hall effect in bilayer electron gas

Spin transport properties of a coupled bilayer electron gas with Rashba spin-orbit coupling are studied. The definition of the spin currents in each layer as well as the corresponding continuity-like equations in the bilayer system are given. The curves of the spin Hall conductivities obtained in each layer exhibit sharp cusps around a particular value of the tunnelling strength and the conductivities undergo sign changes across this point. Our investigation on the impurity effect manifests that an arbitrarily small concentration of nonmagnetic impurities does not suppress the spin Hall conductivity to zero in the bilayer system. Based on these features, an experimental scheme is suggested to detect a magnification of the spin Hall effect.Comment: Revtex 10 pages, 4 figures; largely extended versio

Exotic phase diagram of a topological quantum system

We study the quantum phase transitions (QPTs) in the Kitaev spin model on a triangle-honeycomb lattice. In addition to the ordinary topological QPTs between Abelian and non-Abelian phases, we find new QPTs which can occur between two phases belonging to the same topological class, namely, either two non-Abelian phases with the same Chern number or two Abelian phases with the same Chern number. Such QPTs result from the singular behaviors of the nonlocal spin-spin correlation functions at the critical points.Comment: 10 pages, 5 figure

NIR Calibrations for Soybean Seeds and Soy Food Composition Analysis: Total Carbohydrates, Oil, Proteins and Water Contents [v.2]

Conventional chemical analysis techniques are expensive, time consuming, and often destructive. The non-invasive Near Infrared (NIR) technology was introduced over the last decades for wide-scale, inexpensive chemical analysis of food and crop seed composition (see Williams and Norris, 1987; Wilcox and Cavins, 1995; Buning and Diller, 2000 for reviews of the NIR technique development stage prior to 1998, when Diode Arrays were introduced to NIR). NIR spectroscopic measurements obey Lambert and Beer’s law, and quantitative measurements can be successfully made with high speed and ease of operation. NIR has been used in a great variety of food applications. General applications of products analyzed come from all sectors of the food industry including meats, grains, and dairy products (Shadow, 1998).
Novel NIR calibrations for rapid, reliable and accurate composition analysis of a variety of several soy based foods and bulk soybean seeds were developed and validated in a six-year collaborative project with a large number of different samples (N >~12, 000). The availability of such calibrations is important for establishing NIR as a secondary method for composition analysis of foods and soybeans both in applications and fundamental research

Spin dynamics of two-dimensional electrons with Rashba spin-orbit coupling and electron-electron interactions

We study the spin dynamics of two dimensional electron gases (2DEGs) with Rashba spin-orbit coupling by taking account of electron-electron interactions. The diffusion equations for charge and spin densities are derived by making use of the path-integral approach and the quasiclassical Green's function. Analyzing the effect of the interactions, we show that the spin-relaxation time can be enhanced by the electron-electron interaction in the ballistic regime.Comment: accepted for publication in Phys. Rev.

Fidelity susceptibility in the two-dimensional spin-orbit models

We study the quantum phase transitions in the two-dimensional spin-orbit models in terms of fidelity susceptibility and reduced fidelity susceptibility. An order-to-order phase transition is identified by fidelity susceptibility in the two-dimensional Heisenberg XXZ model with Dzyaloshinsky-Moriya interaction on a square lattice. The finite size scaling of fidelity susceptibility shows a power-law divergence at criticality, which indicates the quantum phase transition is of second order. Two distinct types of quantum phase transitions are witnessed by fidelity susceptibility in Kitaev-Heisenberg model on a hexagonal lattice. We exploit the symmetry of two-dimensional quantum compass model, and obtain a simple analytic expression of reduced fidelity susceptibility. Compared with the derivative of ground-state energy, the fidelity susceptibility is a bit more sensitive to phase transition. The violation of power-law behavior for the scaling of reduced fidelity susceptibility at criticality suggests that the quantum phase transition belongs to a first-order transition. We conclude that fidelity susceptibility and reduced fidelity susceptibility show great advantage to characterize diverse quantum phase transitions in spin-orbit models.Comment: 11 pages. 11 figure